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Title   자궁경부암 환자 부부에서 인유두종 바이러스 감염력 결정을 위한 인유두종 바이러스 16 형 상류전사 조절 부위 염기서열 변체 비교 ( Comparison of HPV 16 Sequence Variations at Upstream Regulatory Region in the Couples of Patients with Cervical Cancer for Determination of HPV Infectivity )
Publicationinfo   1999 Jan; 031(02): 403-411.
Key_word   HPV 16, Variants, Molecular epidemiology
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Abstract   Purpose: Although it is now generally accepted that human papillomaviruses (HPVs) are causally related to cervical neoplasia by plentiful epidemioiogic and experimental works, little is known about the direct evidence of sexual transmission of HPV. This study was undertaken to confirm the transmission route and determine the infectivity of HPV by comparison of HPV 16 sequence variations at upstream regulatory region (URR) in the couples of patient with cervical cancer. Materials and Methods: HPV DNAs obtained from genital lesions of forty married couples of patients with cervical cancer were evaluated by polymerase chain reaction (PCR) and PCR-directed sequencing. Results: HPV 16 was detected in fourteen (63.6%) of twenty-two male consorts whose wives were positive for HPV 16. Of these, six (42.9%) couples demonstrated identical HPV 16 URR variants between patients and male consorts, and eight had mismatching HPV 16 URR sequences. Among six couples showed matching HPV 16 variants, three couples mamed for 10, 19, 25 years respectively carried variant 7728/7779, two couples married for 15 years each carried variant 7728/7762, and one couple married for 18 years carried variant 7728/7797, Conclusion: These data suggest that sexual transmission of HPV 16 does occur. A search for more HPV variants in a large cohort is needed to secure high level of precision in molecular epidemiologic study using HPV variant.
저 자   김재원(Jae Weon Kim),송용상(Yong Sang Song),전혜원(Hye Won Jeon),박노현(No Hyun Park),강순범(Soon Beom Kang),이효표(Hyo Pyo Lee)